FRAP analysis of binding: proper and fitting

作者: B SPRAGUE , J MCNALLY

DOI: 10.1016/J.TCB.2004.12.001

关键词:

摘要: Dynamic molecular interactions are fundamental to all cellular processes. In vivo analyses of these frequently done using fluorescence recovery after photobleaching (FRAP). Proper interpretation FRAP data yields information about the binding fluorescently tagged molecules, including number states and strength each state. This can be gleaned from appropriate models process underlying a recovery. Continued application development approaches promise provide crucial for quantitative description networks that regulate function.

参考文章(64)
Jamie White, Ernst Stelzer, Photobleaching GFP reveals protein dynamics inside live cells Trends in Cell Biology. ,vol. 9, pp. 61- 65 ,(1999) , 10.1016/S0962-8924(98)01433-0
Malte Wachsmuth, Waldemar Waldeck, Jörg Langowski, Anomalous diffusion of fluorescent probes inside living cell nuclei investigated by spatially-resolved fluorescence correlation spectroscopy. Journal of Molecular Biology. ,vol. 298, pp. 677- 689 ,(2000) , 10.1006/JMBI.2000.3692
Douglas Magde, Elliot Elson, W. W. Webb, Thermodynamic Fluctuations in a Reacting System-Measurement by Fluorescence Correlation Spectroscopy Physical Review Letters. ,vol. 29, pp. 705- 708 ,(1972) , 10.1103/PHYSREVLETT.29.705
Alan S Verkman, Solute and macromolecule diffusion in cellular aqueous compartments Trends in Biochemical Sciences. ,vol. 27, pp. 27- 33 ,(2002) , 10.1016/S0968-0004(01)02003-5
D.E. Koppel, D. Axelrod, J. Schlessinger, E.L. Elson, W.W. Webb, Dynamics of fluorescence marker concentration as a probe of mobility Biophysical Journal. ,vol. 16, pp. 1315- 1329 ,(1976) , 10.1016/S0006-3495(76)85776-1
Mu-ming Poo, Richard A. Cone, Lateral diffusion of rhodopsin in the photoreceptor membrane Nature. ,vol. 247, pp. 438- 441 ,(1974) , 10.1038/247438A0
Kubitscheck, Wedekind, Peters, Three‐dimensional diffusion measurements by scanning microphotolysis Journal of Microscopy. ,vol. 192, pp. 126- 138 ,(1998) , 10.1046/J.1365-2818.1998.00406.X
Pascal Farla, Remko Hersmus, Bart Geverts, Pierre O Mari, Alex L Nigg, Hendrikus J Dubbink, Jan Trapman, Adriaan B Houtsmuller, None, The androgen receptor ligand-binding domain stabilizes DNA binding in living cells Journal of Structural Biology. ,vol. 147, pp. 50- 61 ,(2004) , 10.1016/J.JSB.2004.01.002
Bonnie J Howell, Ben Moree, Emily M Farrar, Scott Stewart, Guowei Fang, E.D Salmon, Spindle Checkpoint Protein Dynamics at Kinetochores in Living Cells Current Biology. ,vol. 14, pp. 953- 964 ,(2004) , 10.1016/J.CUB.2004.05.053