DNA Polymorphisms at Bermudagrass Microsatellite Loci and Their Use in Genotype Fingerprinting

摘要: The turf-type bermudagrasses include dip- loid Cynodon transvaalensis Burtt Davy, tetra- ploid C. dactylon (L.) Pers., and sterile triploid hybrids produced by crosses of these species. objective this study was to develop a set microsatellite markers that could distin- guish among commercially important cultivars. A genomic library enriched for the (CA/GT) n repeat motif constructed from DNA 'Tifway' hybrid sequenced identify regions. Twenty-fi ve mic- rosatellite-fl anking primer sets were developed used genotype two plant introductions 12 These an average 10 amplicons across 14 templates. Sequences selected revealed polymorphism resulting expansion and/or contraction micro- satellite indel mutations in fl As few as suffi cient differentiate all unrelated introduction lines failed distinguish closely related culti- vars selection natural variants, but one uniquely distinguished cultivar TifEagle its irradiated parent cul- tivar. microsatellites not derived gene coding sequences will supplement existing expressed sequence tag (EST)-based bermudagrass microsatel- lites. They be most useful evaluating genetic diversity accessions distinguishing cultivars exploit diversity.