Changes in gastric sodium-iodide symporter (NIS) activity are associated with differences in thyroid gland sensitivity to perchlorate during metamorphosis.

摘要: Abstract We investigated stage-dependent changes in sensitivity of the thyroid gland to perchlorate during development African clawed frog tadpoles ( Xenopus laevis ) relation non-thyroidal iodide transporting tissues. Perchlorate-induced increases follicle cell size and colloid depletion were blunted when exposures began at Nieuwkoop–Faber (NF) stage 55 compared NF stages 49 or 1–10. To determine if other tissues may contribute this difference we first examined which expressed transcripts for sodium dependent symporter (NIS). RT-PCR analysis revealed that NIS was stomach small intestine addition X. tadpoles. mRNA not detected lung, kidney, skin, gill, muscle, heart liver. Perchlorate sensitive 125 I uptake found stomach, but liver, heart. Perchlorate-sensitive by 6–10 times greater than any tissue While exhibited perchlorate-sensitive it roughly 4-fold less observed Although abundance gene stomachs from statistically significant. conclude gastric between 55, possibly due post-translational glycosylation trafficking within mucosal cells. These developmental expression affect availability gland.