Oxidative stresses induced the cystine transport activity in human erythrocytes
作者： Yoshinori Ohtsuka , Takahito Kondo , Yoshikazu Kawakami
DOI: 10.1016/S0006-291X(88)81063-5
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摘要: Summary Cystine was transported into human erythrocytes in the presence of tertiary-butyl hydroperoxide (t-BH) or 1-chloro-2,4-dinitrobenzene (CDNB). The transport rate cystine dependent on extracellular concentration t-BH CDNB, and incubation time. According to Dowex-1 column chromatography, incorporated fractions glutathione disulfide (GSSG) glutathione-S (GSH-S) conjugate. competitively inhibited by DL-homocystine alanine. inhibition rates alanine were 75% 68%, with similar Ki values 0.7mM 0.6mM, respectively. It is suggested that induced for synthesis when are exposed oxidative stresses. This system may serve as an emergency function erythrocytes.
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