Increased Erythrocytes By-Products of Arginine Catabolism Are Associated with Hyperglycemia and Could Be Involved in the Pathogenesis of Type 2 Diabetes Mellitus

摘要: Diabetes mellitus (DM) is a worldwide disease characterized by metabolic disturbances, frequently associated with high risk of atherosclerosis and renal nervous system damage. Here, we assessed whether metabolites reflecting oxidative redox state, arginine nitric oxide metabolism, are differentially distributed between serum red blood cells (RBC), significant metabolism exists in RBC. In 90 patients type 2 DM without regular treatment for diabetes healthy controls, paired age gender, measured RBC levels malondialdehyde (MDA), nitrites, ornithine, citrulline, urea. isolated RBC, L-[14C]-arginine was also determined. both groups, nitrites were equally RBC; citrulline predominated serum, whereas urea, arginine, ornithine found mainly showed hyperglycemia increased HbA1C, these metabolites, except significantly correlating glucose levels. observed to be capable catabolizing which from patients, correlated an affinity the activities putative arginase (Km = 0.23±0.06 vs. 0.50±0.13 mM, controls) synthase 0.28±0.06 0.43±0.09 controls). conclusion, our results suggest that alters metabolite distribution demonstrating regulate affect nitrogen not only transporting them but metabolizing amino acids such as arginine. Moreover, confirmed urea can produced human besides hepatocytes, being much more evident DM. These events probably involved specific physiopathology this disease, i.e., endothelial damage dysfunction.