TMEM120A and B: Nuclear Envelope Transmembrane Proteins Important for Adipocyte Differentiation

摘要: Recent work indicates that the nuclear envelope is a major signaling node for cell can influence tissue differentiation processes. Here we present two trans-membrane proteins TMEM120A and TMEM120B are paralogs encoded by Tmem120A Tmem120B genes. The TMEM120 expressed preferentially in fat both induced during 3T3-L1 adipocyte differentiation. Knockdown of one or other protein altered expression several genes required differentiation, Gata3, Fasn, Glut4, while knockdown together additionally affected Pparg Adipoq. double also increased strength effects, reducing example Glut4 levels 95% compared to control cells upon pharmacologically Accordingly, B individually impacted on differentiation/metabolism as measured lipid accumulation through binding Oil Red O coherent anti-Stokes Raman scattering microscopy (CARS). linked lipodystrophies mutations lamin A; however, A widely expressed. Thus it possible fat-specific transmembrane may play contributory role tissue-specific pathology this disorder wider problem obesity.