Red fluorescent protein

摘要: One of the many useful cell biological techniques past few years has been development green fluorescent protein (GFP) as a reporter for gene activity, in which interest and GFP are expressed together. Thus, appearance fluorescence can be used to visualize both expression other its cellular location. Although variants exist with different emission wavelengths, more variety is needed permit multi-fluorescent imaging, or overcome problems inherent tissue fluorescence. The recent publication atomic resolution structure red (RFP) by Mark Wall colleagues [Nat. Struct. Biol. (2000) 7, 1133–1138] should engineering properties so that FPs even gene-reporters. NC