Plant Golgi-associated vesicles contain a novel α-actinin-like protein
作者： Yan Li , Lung-Fei Yen
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摘要: Summary By using Western blotting, immunofluorescence and immuno-gold labeling, a novel α-actinin-like protein was found in pollen tubes of Lilium davidii , model system for cytoskeleton Golgi apparatus study plant. As measured by the molecular mass about 80 kDa. Under confocal laser scanning microscopy after distribution appeared punctated cytoplasm tubes. When double labeled, co-localized with 58K protein. In addition, some fraction to co-distribute F-actin bundles Additional studies labeling transmission electron revealed that bound mainly membranes Golgi-associated vesicles. were treated Brefeldin A (BFA), proteins dispersed into cytoplasm, growth inhibited. After BFA removed, reversibly recovered on apparatus. These results suggest is BFA-sensitive vesicles, may participate vesicles budding and/or sorting, together actin microfilaments.
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