Target specificity of immunoglobulin class switch recombination is not determined by nucleotide sequences of S regions.
作者： Kazuo Kinoshita , Junko Tashiro , Shuhei Tomita , Chung-Gi Lee , Tasuku Honjo
DOI: 10.1016/S1074-7613(00)80650-0
关键词:
摘要: We describe a model system for class switch recombination (CSR) using CH12F3-2 cells transfected with DNA construct containing two S sequences transcribed by different promoters and separated viral thymidine kinase (TK) gene. Recombination observed this shares key properties physiological CSR: deletion of between regions, requirement cytokine stimulation, nonhomologous no consensus breakpoint sequences. Studies on transfectants variants led us to the following conclusions: (1) are required CSR; (2) isotype specificity is not determined nucleotide regions; (3) strand-specific; (4) induction activity requires stimulation.
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