The regulatory network menin-microRNA 26a as a possible target for RNA-based therapy of bone diseases.

摘要: MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression, interplaying with transcription factors in complex regulatory networks. Menin is the product MEN1 oncosuppressor gene, responsible for multiple endocrine neoplasia type 1 syndrome. Recent data suggest that menin functions as a general regulator transcription. expression modulates mesenchymal cell commitment to myogenic or osteogenic lineages. The microRNA 26a (miR-26a) SMAD1 protein during osteoblastic differentiation human adipose tissue-derived stem cells (hADSCs). We used siRNA silencing against mRNA and pre-miR-26 mimics study interplay between them investigate miR-26a hADSCs. found hADSCs siRNA-induced resulted down regulation miR-26a, consequent up-regulation protein. Chromatin immunoprecipitation (ChIP) showed...