Ultrastructural localisation and functional implications of Corticotropin releasing factor, Urocortin and their receptors in cerebellar neuronal development

摘要: The cerebellar circuitry plays a central role in motor coordination and learning. What’s more, the development of is dependent upon cerebellum its circuitry. Thus, an understanding factors influencing integral to elucidating how normal ensues allows for directed interventions cases pathologies. A plethora neuropeptides are expressed within pre-cerebellar centres, which supply afferents. In this thesis, we investigate corticotropin releasing factor (CRF) urocortin developing rat attempt idendtify causal relations between their spatial temporal expression, well-defined stages development. Both CRF initially distributed diffusely throughout at later expression predominates posterior cerebellum, or vestibulo-cerebellum. An important difference that whilst exclusively afferents, localised afferents as well Purkinje neurons, sole output cortex. receptors climbing fibres contacts, cells, suggesting modulate release from effects these peptides on cells once they released. Finally, using organotypic slice cultures, have shown direct differential trophic actions inducing outgrowth differentiation cell dendrites.