Effect of Glutamate on Dendritic Growth in Embryonic Rat Motoneurons

摘要: Glutamate is a major excitatory neurotransmitter for spinal motoneurons. We have investigated its effect on survival and neurite formation in cultures of highly enriched motoneurons from 15-d-old rat embryos. Whereas the these neurons was not reduced by this treatment, distinct specific dendrite outgrowth could be observed. Axon affected glutamate. Our data suggest that calcium influx via ionotropic AMPA/kainate (AMPA/KA) receptors responsible regulation neurotransmission. This shown use inhibitors different classes glutamate receptors. The continuous depolarization at 35 mm KCl treatment with joro spider toxin (JSTX-3, 3 μm), blocker Ca2+-conducting AMPA Removal after 5 d culture led to increased growth during following period, delayed addition resulted reduction length already existing dendrites. observation dose-dependent reversible reflects potential physiological function neurotransmission morphology developmental period when synaptic contacts afferent are made cord.