Follicle-like structure and polarized monolayer: Role of the extracellular matrix on thyroid cell organization in primary culture

摘要: The thyroid follicle, the morphofunctional unit of gland, is a spheroidal structure formed by monolayer polarized cells surrounding closed cavity in which thyroglobulin accumulates. Newly isolated porcine reorganize into two types structures differ orientation cell polarity: follicle-like structures, obtained presence TSH, apical pole delineates and express most parameters characteristic function; inside-out follicles oriented towards culture medium do not properly function. organization newly can be modified stimulation migration or interaction their poles with new environment. Seeded on hard surface (glass, plastic), suspension migrate giving monolayer. On contrary, organized treated hexamethylene bisacetamide, structures. Inside-out reoganize upon collagen I gel, coherent matrix, reconstituted basement membrane (RBM), soft matrix. Overlaid I, monolayers follicles. Embedded RBM, reorient polarity functional RBM surface, pull gel embed themselves matrix finally reorienting to inside-in polarity. When comparing other epithelial (mammary cells, Sertoli cells), it appears that obtention ie organization, best way both morphology function any specific tissue, porous bottom chamber coming just behind.