Structure and Function of ras p21: Studies BY Site-Directed Mutagenesis

摘要: Are studies by analogy fruitful to understanding protein structure and function? With sequences accumulating at an astonishing rate through gene cloning DNA sequencing, a great deal of function can be learned with members the superfamily whose structures have been determined molecular mechanisms action are known. The best case in point, perhaps is unraveling elusive ras p21. model p21 has constructed crystal E. coli elongation factor, EF-Tu (McCormick et al., 1985; Jurnak, 1985). This remarkably consistent actual three dimensional later X-ray crystallography (De Vos 1988). recent identification GAP which stimulates GTPase activity (Trahey McCormick, 1987), conceptual offspring similar biochemical mechanism well understood for synthesis (Kaziro, 1978). Furthermore, characterized G-proteins, regulate transmembrane cell signalling adenylate cyclase systems light transduction retina, forms foundation current belief that mediates transmission growth signals their intracellular effectors control proliferation differentiation (Bourne Sullivan, 1986).