Cereal prolamin evolution and homology revealed by sequence analysis.

摘要: Prolamin mixtures were isolated from oats, rice, normal and high-lysine sorghum, two varieties of pearl millet, strains teosinte, gamma grass subjected to NH2-terminal amino acid sequence determinations. In each case (except for whose prolamins apparently have blocked or unavailable residues), primarily a single was observed despite significant heterogeneity, suggesting that prolamin homology in cereal arose through duplication mutation ancestral gene. Comparisons then made sequences previously determined wheat, corn, barley, rye. Within genera, different subspecies exhibited few differences, but more distantly related subtribes, tribes showed increasingly large differences. the subfamily Festucoideae, no apparent between oats those subtribe Triticinae (including rye, which demonstrated). Panicoideae, corn shown be closely teosinte Tripsacum. Sorghum diverged less than had millet. These comparisons demonstrate analyses can successfully predict clarify evolutionary relationships cereals.