Adipocyte lipolysis in normal weight subjects with obesity among first-degree relatives.

摘要: In this study we investigated whether fat cell lipolysis could be involved in the aetiology of obesity by comparing non-obese subjects with (Hob) or without (Hnorm) a family trait for overweight. A history was present when at least one first-degree relatives had body mass index 27 kg/m2 more. Twenty-seven healthy, drugfree adult were investigated; 13 Hob and remaining 14 Hnorm. Eleven obese parent. Isolated cells from abdominal subcutaneous adipose tissue incubated vitro. Glycerol release (lipolysis index), mRNA levels enzymatic activity hormone-sensitive lipase radioligand binding to beta1- beta2-adrenoceptors determined. The lipolytic effects noradrenaline (major endogenous agent), isoprenaline (a non-selective beta-adrenoceptor agonist), forskolin direct activator adenylyl cyclase) dibutyryl cyclic AMP (activating protein kinase thereby lipase) reduced about 50% (p 0.001 0.01). maximum (p<0.05) correlated responsiveness whole population (r=0.71). However, there no difference between groups steady-state enzyme. Beta1-, beta2- alpha2-adrenoceptor sensitivity as well beta2-adrenoceptor numbers normal Hob. Fasting plasma insulin 49.1 32.6 pmol/l, respectively Hnorm (p=0.01). There was, however, significant correlation vitro insulin. Thus, catecholamine resistance cells, partly due impaired function lipase, is an adipocyte abnormality associated tendency obesity.