INFLUENCE OF HORMONE BALANCE AND IN VITRO PHOTOAUTOTROPHY ON DIANTHUS SPICULIFOLIUS SCHUR MICROPROPAGATION

摘要: 3 Institutul de Cercetări Biologice, str. Republicii, nr. 48, RO-3400 Cluj-Napoca Abstract: The biotechnology of in vitro cultures is increasingly used as part ex situ and programmes for the conservation a number plant species that are endemic, endangered, vulnerable or rare. Dianthus spiculifolius Schur Carpathian endemism. Due to excessive anthropization some regions its distribution area, populations question declining. At present, mentioned various Red Lists elaborated rare Romanian flora (6,9). aseptic culture was induced starting from material harvested Piatra Craiului Massif. inocula consisting apical nodal explants were cultured on media, with varied hormone balance, order obtain an high possible multiplication rate induction rhizogenesis. increased 7.5 new plantlets/inoculum 31.8 plantlets/inoculum, adequate rhizogenesis obtained at same time. Micropropagation acclimatization favored by photoautotrophic culture.