Cytogenetical characterization of UV-sensitive repair-deficient CHO cell line 43-3B II. Induction of cell killing, chromosomal aberrations and sister-chromatid exchanges by 4NQO, mono- and bi-functional alkylating agents
作者： F. Darroudi , A.T. Natarajan , P.H.M. Lohman
DOI: 10.1016/0027-5107(89)90061-4
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摘要: Abstract An established cell line of Chinese hamster ovary (CHO-9) cells and its UV-sensitive mutant 43-3B have been studied for the induction killing, chromosomal aberrations sister-chromatid exchanges (SCEs) after exposure to different types DNA-damaging agents such as 4-nitroquinoline-1-oxide (4NQO), mitomycin C (MMC), diepoxybutane (DEB), methyl methanesulfonate (MMS), ethyl (EMS) nitrosourea (ENU). In comparison with wild-type CHO cells, showed very high sensitivity ot UV-mimetic agent 4NQO DNA cross-linking MMC DEB. The responded higher monofunctional alkylating (MMS, EMS ENU). increased cytotoxic effects all these chemicals correlated well elevated increase in frequency was much than SCEs (4–10-fold) when compared cells. This suggests that are results fundamentally cellular events. responses UV, 4NQO, DEB resemble those 2 human syndromes, i.e., xeroderma pigmentosum Fanconi's anemia. These data suggest defective excision repair other pathways involved cross-links (MMC, DEB) bulky adducts (4NQO).
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