Mechanical disruption of mammalian cells in a microfluidic system and its numerical analysis based on computational fluid dynamics

摘要: The lysis of mammalian cells is an essential part different lab-on-a-chip sample preparation methods, which aim at the release, separation, and subsequent analysis DNA, proteins, or metabolites. Particularly for compartmented in vivometabolism cells, such a method must be very fast compared to metabolic turnover-rates, it should not affect native metabolite concentrations, ideally leave cell organelles undamaged. So far, no available. We have developed microfluidic system effective rapid mechanical disruption established mathematical model describe efficiency system. Chinese hamster ovary (CHO) were disrupted with high by passing through two consecutive micronozzle arrays. Simultaneous compression shearing led rate ≥90% flow Q = 120 μL min−1 per nozzle passage, corresponds mean fluid velocity 13.3 m s−1 Reynolds number 22.6 gap. discussed problem channel clogging cellular debris resulting instability experimental results predictions from Computational Fluid Dynamics (CFD) simulations critical energy dissipation CHO population known size distribution was determined 4.7 × 108 W m−3. Our calculation on basis CFD-data could applied other microgeometries predict intended undesired damage.