Microfluidic devices for high‐throughput proteome analyses

摘要: Over the last decades, microfabricated bioanalytical platforms have gained enormous interest due to their potential revolutionize biological analytics. Their popularity is based on several key properties, such as high flexibility of design, low sample consumption, rapid analysis time, and minimization manual handling steps, which are for proteomics analyses. An ideal totally integrated chip-based microfluidic device could allow automated workflows starting from cell cultivation ending with MS-based proteome analysis. By reducing or eliminating transfer steps increasing throughput analyses these would dramatically improve reliability, reproducibility, proteomic investigations. While complete devices do not exist routine use yet, many improvements been made in translation separation into formats. In this review, we will focus recent developments strategies enable integrate devices.