TIRF-based biosensor for sensitive detection of progesterone in milk based on ultra-sensitive progesterone detection in water.

摘要: We report on recent advances of our immunoassay for the hormone progesterone in cow’s milk. Detection is based total internal reflectance fluorescence (TIRF), binding-inhibition assay with an immobilized derivative, and a commercially available monoclonal antibody to as biological recognition element. The fully automated River Analyzer (RIANA) biosensor unattended, cost-effective, continuous monitoring environmental pollution therefore was adapted sensitive determination First, sensitivity robustness existing water analysis were improved, resulting detection limit (LOD) only 0.2 pg mL−1 quantification (LOQ) 2.0 mL−1. These extraordinary results are lowest limits using biosensors yet reported literature. Second, accurate indicator ovulation calibrated detected three different types milk (UHT milk, fresh raw milk). For commercial randomly procured nominal levels typically range 5–15 ng Limits achieved added (i.e. spiked samples) between 45.5 56.1 depending type. Having mind 1:10 dilution factor, these still success. first time incorporated into bovine giving below 1 biosensor. Thus outstanding progress made this has now been successfully use field reproduction management.