Optimization of the Synthesis of Structured Phosphatidylcholine with Medium Chain Fatty Acid.

摘要: Structured phosphatidylcholine was successfully produced by acidolysis between and free medium chain fatty acid, using phospholipase A1 immobilized on Duolite A568. Response surface methodology applied to optimize the reaction system three process parameters: molar ratio of substrates (phosphatidylcholine acid), enzyme loading, temperature. All parameters evaluated showed linear quadratic significant effects production modified phosphatidylcholine; contributed positively, but temperature influenced negatively. Increased loading also led increased only during first 9 hours reaction. Optimal conditions obtained from model were a acid 1:15, an 12%, 45°C. Under these 52.98 % after 24 h The prediction confirmed verification experiments; 53.02%, total yield 64.28% incorporation 42.31%. scaled-up in batch reactor with similar phosphatidylcholine, acid. Purification column chromatography structured yielded 62.53% enriched 42.52%