Rapid and high yield production of phospholipids enriched in CLA via acidolysis: The critical role of the enzyme immobilization protocol
作者： Carlos M. Verdasco-Martín , Carlos Corchado-Lopo , Roberto Fernández-Lafuente , Cristina Otero
DOI: 10.1016/J.FOODCHEM.2019.05.107
关键词: Yield (chemistry) 、 Hydrolysis 、 Linolenic acid 、 Phosphatidylcholine 、 Conjugated system 、 Food grade 、 Chemistry 、 Immobilized enzyme 、 Styrene 、 Chromatography 、 Food science 、 Analytical chemistry 、 General Medicine
摘要: Abstract Phospholipids (PL) rich in conjugated linolenic acid (CLA) have important health effects. Yields of phosphatidylcholine (PC) acidolysis with CLA use to be limited to In this study, PC hydrolysis has been practically eliminated using extremely dried lyophilized (279 ± 4 mg water/Kg PC), obtaining close 100% molar yield modified (72.3% CLA) Duolite-Lecitase 24 h, the highest reported literature for reaction. It better improved by changing immobilization support, three food grade hydrophobic supports (Styrene, and two Octadecyl methacrylates (OM OMC)). only 2 h, a 1/12 PC/CLA ratio at 50 °C, similar almost quantitative yields 74.4% content obtained OM-Lecitase. The fatty composition PCs is not affected enzyme protocol.
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