Hyperthermia enhances the cytotoxicity and platinum-DNA adduct formation of lobaplatin and oxaliplatin in cultured SW 1573 cells

摘要: The cytotoxicity of cisplatin and cisplatin-DNA adduct formation in vitro vivo is clearly enhanced by hyperthermia. We investigated whether platinum-DNA two promising new third-generation platinum derivatives, lobaplatin [1,2-diamminomethylcyclobutane platinum(II) lactate] oxaliplatin [oxalato-1,2-diaminocyclohexane platinum(II)], are also Cisplatin was used for comparison. SW 1573 cells were incubated with cisplatin, or at different concentrations 1 h 37°, 41° 43°C. reproductive capacity determined cloning experiments. Immunocytochemical detection adducts performed the rabbit antiserum NKI-A59. At 37°C, most cytotoxic, followed lobaplatin. Hyperthermia oxaliplatin. There no further increase 43°C compared to 41°C A observed thermal enhancement higher than oxaliplatin, reverse pattern 41°C. For both drugs, lower cisplatin. feasible all drugs. Adduct a relative 410%, 170% 180%. These results seem confirm that an involved cytotoxicity. warrants investigations.