Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography
作者： Chyongere Hsieh , Thomas Schmelzer , Gregory Kishchenko , Terence Wagenknecht , Michael Marko
DOI: 10.1016/J.JSB.2013.10.019
关键词:
摘要: … by cryo-transmission electron microscopy (cryo-TEM), which is … too thick to study intact in the cryo-TEM. Cryo focused-ion-… of loading the specimen into the cryo-TEM. We present a robust …
elsevier.com
sci-hub.se 参考文章(33)
Michael Marko, Chyong-Ere Hsieh, Carmen A. Mannella, Electron Tomography of Frozen-hydrated Sections of Cells and Tissues Springer, New York, NY. pp. 49- 81 ,(2007) , 10.1007/978-0-387-69008-7_3
J. Frank, Three-dimensional electron microscopy of macromolecular assemblies : visualization of biological molecules in their native state Oxford University Press. ,(2005)
Müller M, Michel M, Studer D, High pressure freezing comes of age Scanning microscopy. ,vol. 3, pp. 253- 269 ,(1989)
Alexander Rigort, Elizabeth Villa, Felix J.B. Bäuerlein, Benjamin D. Engel, Jürgen M. Plitzko, Integrative Approaches for Cellular Cryo-electron Tomography Methods in Cell Biology. ,vol. 111, pp. 259- 281 ,(2012) , 10.1016/B978-0-12-416026-2.00014-5
K.M. STRUNK, K. WANG, D. KE, J.L. GRAY, P. ZHANG, Thinning of large mammalian cells for cryo-TEM characterization by cryo-FIB milling. Journal of Microscopy. ,vol. 247, pp. 220- 227 ,(2012) , 10.1111/J.1365-2818.2012.03635.X
Oleg Kuybeda, Gabriel A. Frank, Alberto Bartesaghi, Mario Borgnia, Sriram Subramaniam, Guillermo Sapiro, A collaborative framework for 3D alignment and classification of heterogeneous subvolumes in cryo-electron tomography Journal of Structural Biology. ,vol. 181, pp. 116- 127 ,(2013) , 10.1016/J.JSB.2012.10.010
Ashraf Al-Amoudi, Lars P.O. Norlen, Jacques Dubochet, Cryo-electron microscopy of vitreous sections of native biological cells and tissues Journal of Structural Biology. ,vol. 148, pp. 131- 135 ,(2004) , 10.1016/J.JSB.2004.03.010
Alexander Rigort, Felix J.B. Bäuerlein, Andrew Leis, Manuela Gruska, Christian Hoffmann, Tim Laugks, Ulrike Böhm, Matthias Eibauer, Helmut Gnaegi, Wolfgang Baumeister, Jürgen M. Plitzko, Micromachining tools and correlative approaches for cellular cryo-electron tomography Journal of Structural Biology. ,vol. 172, pp. 169- 179 ,(2010) , 10.1016/J.JSB.2010.02.011
DA de Winter, Rob J Mesman, Michael F Hayles, Chris TWM Schneijdenberg, Cliff Mathisen, Jan A Post, None, In-situ integrity control of frozen-hydrated, vitreous lamellas prepared by the cryo-focused ion beam-scanning electron microscope. Journal of Structural Biology. ,vol. 183, pp. 11- 18 ,(2013) , 10.1016/J.JSB.2013.05.016
Ke Wang, Korrinn Strunk, Gongpu Zhao, Jennifer L. Gray, Peijun Zhang, 3D structure determination of native mammalian cells using cryo-FIB and cryo-electron tomography. Journal of Structural Biology. ,vol. 180, pp. 318- 326 ,(2012) , 10.1016/J.JSB.2012.07.003