Initiation factor eIF-4E of Saccharomyces cerevisiae. Distribution within the cell, binding to mRNA, and consequences of its overproduction.

摘要: The eukaryotic translational initiation factor 4E (eIF-4E) is an essential protein that binds the 5' cap structure with high specificity and affinity. Yeast eIF-4E homologous to of higher eukaryotes, but interacts a different set cap-binding complex proteins. In present study distribution yeast in Saccharomyces cerevisiae was found be similar observed cells, whereby more concentrated nucleus than cytoplasm. Overexpression S. exerted at most minimal effect on growth liquid medium not influence translation reporter gene mRNAs bearing secondary their leader regions. new method mRNA-protein interactions, biotinylated were synthesized vitro for use studies binding extracts. Streptavidin used adsorb plus bound factors. Stem-loop structures region did or, comparative experiments, eIF-4A. Thus shows both similarities differences respect function its counterparts eukaryotes.