eIF4E isoform 2 in Schizosaccharomyces pombe is a novel stress‐response factor

摘要: Cap-binding proteins of the eIF4E family are generally involved in mediating ribosome recruitment to capped mRNA via an interaction with initiation factor eIF4G. However, Schizosaccharomyces pombe has two isoforms, one which (eIF4E2, encoded by tif452) a relatively low affinity for We show that tif452 is required specific stress responses. An S. pombe, tif452Δ mutant manifests slow growth under conditions nutrient, temperature and salt stress. eIF4E2 shows distinct subcellular distribution eIF4E1, cap-binding mainstream translation. In response stress, cellular level increases, whereas amount intact eIF4G decreases, leaving as predominant isoform cell deficient The presence modifies competence ribosomes translate mRNAs structured leaders vivo. promoter putative stress-response (T-rich) motifs, seems be new type factor.