Generation, persistence, and modulation of Th0 effector cells: role of autocrine IL-4 and IFN-gamma.

摘要: Many studies have classified CD4 responses into either Th1-like or Th2-like, based on cytokine secretion profiles, but little significance has been placed Th0 cells. This largely resulted from that suggested populations primarily comprise individual Th1 and Th2 Here, we show priming of Ag-specific naive cells with moderate dose IL-4 generates a population is evident after 3 days in vitro becomes prevalent successive encounters Ag over 9-day period. By intracellular staining, the majority (>60%) effector generated this way produce IL-4, IFN-γ IL-2, without IL-2. Endogenous secreted initial culture was critical for generating cells, since neutralization allowed to induce differentiation Th2-like Successive were required their stability persistence governed by balance endogenous during later stages differentiation. Studies blocking Fas-induced cell death showed process played no role generation, differential committed not persistence. These data suggest can be as Th1- activation, relative levels autocrine are important lack commitment, all predestined phenotypes early response.