Transforming growth factor-beta and IL-4 cause helper T cell precursors to develop into distinct effector helper cells that differ in lymphokine secretion pattern and cell surface phenotype.

摘要: We have investigated the effects of TGF-beta on in vitro development different subsets Th cells and find that addition results generation cell populations with distinct characteristics resemble those memory cells. Resting, short-lived CD4+ precursor T can be induced by mitogen stimulation to proliferate differentiate cultures after 4 7 days will generate a population that, when restimulated, synthesize secrete high titers wide variety lymphokines. It has been previously reported presence lymphokine IL-4 during culture "effector" rapidly IL-4, IL-5, IL-3, IFN-gamma, granulocyte-macrophage-CSF. added precursors, suppresses IL-4/IL-5 secreting effectors instead IL-2 IFN-gamma. CD4 generated show little or no expression CD45RB, contrast developed (or alone) express surface densities CD45RB. The kinetics recovery also differs rather than is present culture. Cultures TGF-beta, initially low recoveries but expand dramatically between whereas induces optimum at day decrease further properties grown thus several attributes common highly differentiated cells, i.e., as predominant cytokine, easy propagation Therefore, we propose hypothesis favors population(s) likely give rise although effector secretes Th2