Using TRIP for genome-wide position effect analysis in cultured cells.
作者： Waseem Akhtar , Alexey V Pindyurin , Johann de Jong , Ludo Pagie , Jelle ten Hoeve
关键词:
摘要: A detailed protocol for analyzing thousands of reporters integrated in parallel (TRIP) at a genome-wide level to study the influence local chromatin context on gene expression.
nature.com
sci-hub.se 参考文章(30)
Jack R. Girton, Kristen M. Johansen, Chromatin structure and the regulation of gene expression: the lessons of PEV in Drosophila. Advances in Genetics. ,vol. 61, pp. 1- 43 ,(2008) , 10.1016/S0065-2660(07)00001-6
EK-W Hui, P-C Wang, SJ Lo, None, Strategies for cloning unknown cellular flanking DNA sequences from foreign integrants. Cellular and Molecular Life Sciences. ,vol. 54, pp. 1403- 1411 ,(1998) , 10.1007/S000180050262
Rebecca S. Devon, David J. Porteous, Anthony J. Brookes, Splinkerettes—improved vectorettes for greater efficiency in PCR walking Nucleic Acids Research. ,vol. 23, pp. 1644- 1645 ,(1995) , 10.1093/NAR/23.9.1644
Katrin Voigt, Andreas Gogol-Döring, Csaba Miskey, Wei Chen, Toni Cathomen, Zsuzsanna Izsvák, Zoltán Ivics, Retargeting Sleeping Beauty Transposon Insertions by Engineered Zinc Finger DNA-binding Domains Molecular Therapy. ,vol. 20, pp. 1852- 1862 ,(2012) , 10.1038/MT.2012.126
Vladimir N Babenko, Igor V Makunin, Irina V Brusentsova, Elena S Belyaeva, Daniil A Maksimov, Stepan N Belyakin, Peter Maroy, Lyubov A Vasil'eva, Igor F Zhimulev, None, Paucity and preferential suppression of transgenes in late replication domains of the D. melanogaster genome BMC Genomics. ,vol. 11, pp. 318- 318 ,(2010) , 10.1186/1471-2164-11-318
Sandra Ruf, Orsolya Symmons, Veli Vural Uslu, Dirk Dolle, Chloé Hot, Laurence Ettwiller, François Spitz, Large-scale analysis of the regulatory architecture of the mouse genome with a transposon-associated sensor Nature Genetics. ,vol. 43, pp. 379- 386 ,(2011) , 10.1038/NG.790
Waseem Akhtar, Johann de Jong, Alexey V. Pindyurin, Ludo Pagie, Wouter Meuleman, Jeroen de Ridder, Anton Berns, Lodewyk F.A. Wessels, Maarten van Lohuizen, Bas van Steensel, Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel Cell. ,vol. 154, pp. 914- 927 ,(2013) , 10.1016/J.CELL.2013.07.018
Trevor J. McFarland, Yi Zhang, La-ongsri Atchaneeyaskul, Peter Francis, J. Timothy Stout, Binoy Appukuttan, Evaluation of a novel short polyadenylation signal as an alternative to the SV40 polyadenylation signal Plasmid. ,vol. 56, pp. 62- 67 ,(2006) , 10.1016/J.PLASMID.2005.11.005
R. Saiki, D. Gelfand, S Stoffel, S. Scharf, R Higuchi, G. Horn, K. Mullis, H. Erlich, Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase Science. ,vol. 239, pp. 487- 491 ,(1988) , 10.1126/SCIENCE.2448875
Frank Weber, Jean de Villiers, Walter Schaffner, An SV40 “enhancer trap” incorporates exogenous enhancers or generates enhancers from its own sequences Cell. ,vol. 36, pp. 983- 992 ,(1984) , 10.1016/0092-8674(84)90048-5