An SV40 “enhancer trap” incorporates exogenous enhancers or generates enhancers from its own sequences

摘要: Abstract We have transfected monkey CV-1 cells with non-infectious, linear SV40 DNA, lacking the 72 by repeat enhancer region. Infectious virus was recovered from this "enhancer trap" upon cotransfection DNA segments various viruses, notably a truncated polyoma that integrated as dimer. Cotransfection of fragmented mouse, monkey, or human origin yielded no recombinant cellular sequences, one possible exception. In some transfection experiments without added viral variants were generated segment their flanking "late" duplicated to substitute for deleted repeat. variant, an 88 duplication creates strong nonenhancing Both fragment and spontaneously created enhancers lack alternating purines-pyrimidines "CACA box" suggested be characteristic elements show only limited homology "GTGG(AAATTT)G box."