miR-15/16 Restrain Memory T Cell Differentiation, Cell Cycle, and Survival

摘要: Coordinate control of T cell proliferation, survival, and differentiation are essential for host protection from pathogens cancer. Long-lived memory cells, whose precursors formed during the initial immunological insult, provide future encounters, their generation is goal many vaccination strategies. microRNAs (miRNAs) key nodes in regulatory networks that shape effective responses through fine-tuning thousands genes. Here, using compound conditional mutant mice to eliminate miR-15/16 family miRNAs T cells, we show restrict cycle, differentiation. High throughput sequencing RNA isolated by cross-linking immunoprecipitation AGO2 combined with gene expression analysis miR-15/16-deficient T cells indicates these effects mediated direct inhibition an extensive network target genes within pathways critical cell memory.