Subunit composition and quantitative importance of GABA(A) receptor subtypes in the cerebellum of mouse and rat.

摘要: In cerebellum, 13 different GABAA receptor subunits are expressed. The number of subtypes formed in this tissue, their subunit composition and quantitative importance so far has not been determined. the present study, immunodepletion by immunoaffinity chromatography, as well immunoprecipitation western blot analysis was performed using subunit-specific antibodies to provide an overview on abundance mouse rat cerebellum. Results obtained indicate that α1βxγ2, α1α6βxγ2, α6βxγ2, α6βxδ α1α6βxδ major addition, small amounts α1βxδ receptors a series minor containing α2, α3, α4, α5, γ1 or γ3 also Whereas is other quite similar these tissues. Data for first time cerebellum represent basis further studies investigating changes expression under pathological conditions.