Quantitative Characterization of α6 and α1α6 Subunit-containing Native γ-Aminobutyric AcidA Receptors of Adult Rat Cerebellum Demonstrates Two α Subunits per Receptor Oligomer
作者： Simon Pollard , Christopher L. Thompson , F. Anne Stephenson
关键词: Copurification 、 GABAA receptor 、 Biochemistry 、 Molecular biology 、 Protein subunit 、 Population 、 Interleukin 10 receptor, alpha subunit 、 Receptor 、 Interleukin 5 receptor alpha subunit 、 Biology 、 Cys-loop receptors
摘要: γ-Aminobutyric acidA (GABAA) receptors were purified from adult rat cerebella by anti-α6(1-16 Cys) antibody affinity chromatography. Immunoblots of the α6 subunit-containing showed copurification α1, β2/3, γ2, δ but not α2 and α3 GABAA receptor polypeptides. Further fractionation this subpopulation anti-GABAA subunit α6(1-16 anti-α1(413-429) columns in series substantiated coassociation α1 The percentage coexistence two subunits was determined quantitative immunoblotting, which found that 41 ± 12% immunoreactivity is associated with subunit. ratios α1:α6 double preparations to be 1:1, thus determining directly for first time within native receptors. benzodiazepine pharmacology α1α6 shown predominantly benzodiazepine-insensitive immunoprecipitation assays. These results are direct studies a population
sci-hub.se 参考文章(24)
F A Stephenson, THE GABAA RECEPTORS Biochemical Journal. ,vol. 310, pp. 1- 9 ,(1995) , 10.1042/BJ3100001
R M McKernan, C I Ragan, N P Gillard, K Quirk, P J Whiting, Model of subunit composition of gamma-aminobutyric acid A receptor subtypes expressed in rat cerebellum with respect to their alpha and gamma/delta subunits. Journal of Biological Chemistry. ,vol. 269, pp. 16020- 16028 ,(1994) , 10.1016/S0021-9258(17)33967-4
S. Pollard, M.J. Duggan, F.A. Stephenson, Further evidence for the existence of alpha subunit heterogeneity within discrete gamma-aminobutyric acidA receptor subpopulations. Journal of Biological Chemistry. ,vol. 268, pp. 3753- 3757 ,(1993) , 10.1016/S0021-9258(18)53758-3
F A Stephenson, M J Duggan, S Pollard, The gamma 2 subunit is an integral component of the gamma-aminobutyric acidA receptor but the alpha 1 polypeptide is the principal site of the agonist benzodiazepine photoaffinity labeling reaction. Journal of Biological Chemistry. ,vol. 265, pp. 21160- 21165 ,(1990) , 10.1016/S0021-9258(17)45340-3
H A Wieland, H Lüddens, P H Seeburg, A single histidine in GABAA receptors is essential for benzodiazepine agonist binding. Journal of Biological Chemistry. ,vol. 267, pp. 1426- 1429 ,(1992) , 10.1016/S0021-9258(18)45961-3
D Benke, S Mertens, A Trzeciak, D Gillessen, H Mohler, GABAA receptors display association of γ2-subunit with α1- and β2/3-subunits Journal of Biological Chemistry. ,vol. 266, pp. 4478- 4483 ,(1991) , 10.1016/S0021-9258(20)64347-2
S. Mertens, D. Benke, H. Mohler, GABAA receptor populations with novel subunit combinations and drug binding profiles identified in brain by alpha 5- and delta-subunit-specific immunopurification. Journal of Biological Chemistry. ,vol. 268, pp. 5965- 5973 ,(1993) , 10.1016/S0021-9258(18)53413-X
M.J. Duggan, S. Pollard, F.A. Stephenson, Immunoaffinity purification of GABAA receptor alpha-subunit iso-oligomers. Demonstration of receptor populations containing alpha 1 alpha 2, alpha 1 alpha 3, and alpha 2 alpha 3 subunit pairs. Journal of Biological Chemistry. ,vol. 266, pp. 24778- 24784 ,(1991) , 10.1016/S0021-9258(18)54297-6
Christopher L. Thompson, Geert Bodewitz, F.Anne Stephenson, Jonathan D. Turner, Mapping of GABAA receptor α5 and α6 subunit-like immunoreactivity in rat brain Neuroscience Letters. ,vol. 144, pp. 53- 56 ,(1992) , 10.1016/0304-3940(92)90714-I
Ruth M. McKernan, Kathleen Quirk, Richard Prince, Paul A. Cox, Nigel P. Gillard, C.Ian Ragan, Paul Whiting, GABAA receptor subtypes immunopurified from rat brain with α subunit-specific antibodies have unique pharmacological properties Neuron. ,vol. 7, pp. 667- 676 ,(1991) , 10.1016/0896-6273(91)90379-E