Model of subunit composition of gamma-aminobutyric acid A receptor subtypes expressed in rat cerebellum with respect to their alpha and gamma/delta subunits.

摘要: Antibodies specific for subunits of the gamma-aminobutyric acid A (GABAA) receptor have been used to immunoprecipitate [3H]muscimol, [3H]Ro 15-4513, and 15-1788 binding sites from deoxycholate-solubilized preparations rat cerebellum. Of antisera raised against alpha subunits, those 6 immunoprecipitated largest proportion receptors. Two populations 6-containing GABAA receptors were identified. The first was labeled with 15-4513 exhibited a pharmacological profile consistent that observed beta 2 gamma in transfected cells (Luddens, H., Pritchett, D. B., Kohler, M., Killisch, I., Keinanen, K., Monyer, Sprengel, R., Seeberg, P. H. (1990) Nature 346, 648-651). second population only [3H]muscimol deduced, quantitative immunoprecipitation studies using combinations antibodies, contain both delta subunits. subunit not be present combination other or 1 subunit. Each found one Some (alpha 4, 5, 3) detectable. By combining information experiments Western blot analysis, model describing composition all cerebellum constructed defined following gamma/delta (percentage cerebellar receptors): (36%), (23%), (28%), (8%), 3 (5%).