pH Dependence of Chitosan Enzymolysis

摘要: As a means of making chitosan more useful in biotechnological applications, it was hydrolyzed using pepsin, chitosanase and α-amylase. The enzymolysis behavior these enzymes further systematically studied for its effectiveness the production low-molecular-weight chitosans (LMWCs) other derivatives. study showed that depend on ion hydronium (H3O+), thus pH with dependence fitting R2 value 0.99. In y = 1.484[H^+] + 0.114, equation dependence, when [H^+] increases by one, (k_0/k_m) 1.484. From temperature study, activation energy (Ea) pre-exponential factor (A) were almost identical two enzymes, but considerable difference observed comparison third enzyme. Chitosanase pepsin had nearly Ea, α-amylase significantly lower. This serves as evidence hydrolysis reaction relies low-barrier hydrogen bonds (LBHBs), which explains low Ea actual conditions. confirmation this phenomenon derived from similarly order magnitudes A between than five. Variation rate constants enzymatic follows Arrhenius equation.