A DEAD-box RNA helicase produces two forms of transcript that differentially respond to cold stress in a cryophyte (Chorispora bungeana).

摘要: This work demonstrated that a cold-induced DEAD-box RNA helicase, CbDRH, is also post-transcriptionally regulated upon cold stress, and it interacts with cold-responsive, glycine-rich, RNA-binding protein, CbGRP. Chorispora bungeana (C. bungeana) representative alpine subnival plant species shows strong tolerance to multiple abiotic stresses, especially stress. helicases are implicated in almost all metabolic processes participate stress responses. Here, we characterized helicase gene from C. bungeana. We cloned the full-length cDNA of by RACE called Helicase (CbDRH). Structurally, CbDRH possesses nine conserved motifs characteristic protein family members its central region, N- C- terminal extensions both harbor glycine-rich region containing several RGG-box motifs. The produces two forms transcripts, CbDRH.2 CbDRH.1, alternative splicing. comes complete excision introns, while CbDRH.1 results use an 5′ splice site eighth intron, retaining part intron (the first 260 bp) early stop codon. Semi-quantitative RT-PCR analysis showed CbDRH.2, but not up-regulated However, abundance transcript can be elevated cycloheximide (an inhibitor nonsense-mediated decay) treatment, indicating targeted decay (NMD). A subcellular localization located nuclei. Further investigation suggested interact CbGRP (Chorispora protein). These data suggest under may function together CbGRP, response.