HCN1 subunits contribute to the kinetics of Ih in neonatal cortical plate neurons

摘要: The distribution of ion channels in neurons regulates neuronal activity and proper formation networks during development. One the is hyperpolarization-activated cyclic nucleotide-gated (HCN) channel constituting molecular substrate current (Ih). Our previous study implied a role for fastest activating subunit HCN1 generation Ih rat neonatal cortical plate neurons. To better understand impact early neocortical development, we here performed biochemical analysis whole-cell recordings juvenile layer 5 somatosensory HCN1−/− control HCN1+/+ mice. Western Blot revealed that protein expression tissue HCN+/+ mice amounted to only 3% young adult cortex suggested other isoforms (particularly HCN4) might be compensatory up-regulated. At first day after birth, functional ablation did not affect proportion expressing pyramidal Although contribution individual proteins remains open, lack markedly slowed activation deactivation However, it impair maximal amplitude/density, voltage dependence activation, cAMP sensitivity. In conclusion, our data imply that, although relatively low, contributes substantially properties These are significantly changed HCN1−/−, either due itself or mechanisms. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 73: 785–797,