Tissue-specific expression of keratin proteins in human esophageal and epidermal epithelium and their cultured keratinocytes☆

摘要: In contrast to the simplified keratin content of bovine, rabbit, and rat esophageal epithelium (composed mainly a 57 46 or 51 kD keratin, depending on animal species), human contained quantitatively different array proteins, ranging in molecular weight from 37 61 kD. The pattern proteins differed qualitatively that epidermis. Human lacked 63, 65, 67 keratins characteristic epidermis, consistent with absence granular layer an anucleate stratum corneum. Moreover, distinctive protein which was either not present only small amounts epidermis variable keratin. Whereas 56, 59, were most abundant 52, 57, predominated epithelium. During vitro cultivation, both epidermal keratinocytes produce colonies are stratified, but morphologic appearance these cultured epithelia differs. Only contain keratohyalin granules outermost layers prominent immunoprecipitation. Otherwise contents appear similar. conclusion, exhibited intertissue interspecies differences proteins. retained some aspects their program differentiation.