Geminin and Brahma act antagonistically to regulate EGFR–Ras–MAPK signaling in Drosophila

摘要: Geminin was identified in Xenopus as a dual function protein involved the regulation of DNA replication and neural differentiation. In Xenopus, acts to antagonize Brahma (Brm) chromatin-remodeling protein, Brg1, during Here, we investigate interaction with Brm complex Drosophila development. We demonstrate that (Gem) interacts antagonistically Brm–BAP wing Moreover, show vivo development biochemically promote EGFR–Ras–MAPK signaling, indicated by its effects on pERK levels, while Gem opposes this. Furthermore, gem brm alleles modulate phenotype Raf gain-of-function mutant eye EGFR mutant. Western analysis revealed over-expression background compromised for reduces Mek (MAPKK/Sor) consistent decrease ERK activation observed. Taken together, our results act signaling pathway, affecting levels