Cloning of bovine GAP and its interaction with oncogenic ras p21
作者： Ursula S. Vogel , Richard A. F. Dixon , Michael D. Schaber , Ronald E. Diehl , Mark S. Marshall
DOI: 10.1038/335090A0
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摘要: The plasma membrane-bound mammalian ras proteins of relative molecular mass 21,000 (ras p21) share biochemical and structural properties with other guanine nucleotide-binding regulatory (G-proteins)1–3. Oncogenic p21 variants result from amino acid substitutions at specific positions that cause to occur predominantly complexed GTP in vivo. Recently, a GTPase activating protein (GAP) cytosolic activity has been discovered stimulates the normal but not oncogenic (ref. 4). GAP might be either negative agent which acts further upstream pathway or downstream target (refs 3, 5 6). We have identified bovine brain apparent 125,000 activity7. Here, using pure kinetic competition assay, we show interacts preferentially active complexes both Harvey (Ha) compared inactive GDP complexes. also report cloning sequencing complementary DNA for GAP. Regions similarity noncatalytic domain adenylate cyclase yeast Saccharomyces cerevisiae8–10 regions conserved between phospholipase C-148, crk oncogene product nonreceptor tyrosine kinases26,27.
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