Continuous glucose monitoring in interstitial fluid using glucose oxidase-based sensor compared to established blood glucose measurement in rats.

摘要: Abstract Glucose monitoring is of importance for success complex therapeutic interventions in diabetic patients. Its impact on treatment and glycemic control demonstrated large clinical trials. Up to eight blood glucose measurements per day are recommended. Notwithstanding, a substantial number patients cannot or will not monitor their appropriately. Considerable progress disturbed metabolism can be expected by continuous monitoring. The aim the study was evaluate performance new amperometric oxidase-based sensor vitro vivo after subcutaneous implantation into rats. For testing current output sensors measured exposure increasing decreasing concentrations up 472 mg dL −1 over time period 7 days. After interscapular region male rats interstitial fluid evaluated compared arterial Hyper- hypoglycaemia were induced intravenous application insulin, respectively. Current each implanted converted concentration using first measurement only. A change with 0.35 nA mg  dL indicates high sensitivity vitro. response (90% steady state) calculated approximately 60 s. Test strips as reference readings found an appropriate rapidly available method comparison established hexokinase automated lab analyzer limits agreement +32.8 −25.7 mg dL Bland-Altman analysis. In normo- hypoglycaemic range correlated well whereas hyperglycaemia reflected completely when changing rapidly. data given characterize sensitivity, long term stability short time. single calibration required only periods findings demonstrate that highly promising candidate assessment humans.