The M1 gene is associated with differences in the temperature optimum of the transcriptase activity in reovirus core particles.

摘要: The reovirus core is a multienzyme complex that contains five different structural proteins and 10 segments of double-stranded RNA. responsible for transcribing mRNA from the enclosed transcriptase has an unusual temperature profile, with optimum transcription occurring at approximately 50 degrees C little activity below 30 or above 60 C. Purified serotype 1 Lang (T1L) cores transcribed most efficiently 48 purified 3 Dearing (T3D) was 52 In addition, T1L produced more per particle than did T3D their respective optima. Core particles were x reassortants used to map these differences. M1 gene, which encodes minor protein mu 2, uniquely associated difference in (P = 0.0003). L1 lambda (previously implicated as RNA polymerase), gene absolute amounts transcript 0.01 P 0.0002, respectively). These data suggest 2 also plays role transcription.