The Reovirus Mutant tsA279 Has Temperature-Sensitive Lesions in the M2 and L2 Genes: The M2 Gene Is Associated with Decreased Viral Protein Production and Blockade in Transmembrane Transport

摘要: Temperature-sensitive mutants provide an ideal means for dissecting viral assembly pathways. The morphological variants produced by end biological characteristics of tsA279, a previously uncharacterized mutant from the Fields' panel temperature-sensitive reovirus, were determined under restrictive growth conditions. showed distinctive pattern increased temperature sensitivity as was raised 39° to 40°. Wild-type reovirus type 1 Lang and crossed generate reassortants. Efficiency plating analyses reassortants that tsA279 has lesions in two genes, mildly one L2, which encodes core spike protein λ2, stronger, dominant lesion M2, major outer capsid μ1. Electron microscopic examination thin-sectioned -infected cells three ways phenotypes expressed. appeared be blocked transmembrane transport virions, phenotype mapped M2 gene; significantly reduced amounts identifiable particles; those particles variants. Immunofluorescent microscopy Immunoprecipitations tsA279- various T1L × reassortant-infected suggested reduction observed progeny caused decreased production proteins at nonpermissive temperature. This also gene.