Fluorescein diacetate and ethidium bromide staining to determine the viability of Mycobacterium smegmatis and Escherichia coli.

摘要: The ability of the fluorescein diacetate and ethidium bromide fluorescent staining method to assess percentage viable bacterial cells in suspension was compared with plate counting method. Mycobacterium smegmatis Escherichia coli cell suspensions were incubated at 60 degrees C. At different time intervals samples taken each sample assessed by showed a positive correlation However, counts lower than when C, indicating lag period decay enzymes after death. Hence, technique can be used trend death rather exact number bacilli.