Effect of recombinant adenovirus vector mediated human interleukin-24 gene transfection on pancreatic carcinoma growth.

摘要: BACKGROUND Pancreatic cancer is a highly malignant tumor affecting an ever increasing number of patients with mean 5-year survival rate below 4%. Therefore, gene therapy for has become potential novel therapeutic modality. In this study we sought to determine the inhibitory effects adenovirus-mediated human interleukin-24 (AdhIL-24) on pancreatic cancer. METHODS Human was cloned into replication-defective adenovirus specific patu8988 cells by virus recombination technology. Reverse transcription-polymerase chain reaction and Western blotting analysis were used expression mRNA in vitro. Induction apoptosis overexpression determined flow cytometry. vivo efficacy adenoviral delivery assessed nude mice (n = 10 each group) bearing cell lines determining inhibition growth, endothelial growth factor CD34 expression, intratumoral microvessel density (MVD). RESULTS The recombinant vector AdVGFP/IL-24 constructed packaged retrovirus titer 1.0 x 10(10) pfu/ml successfully expressed both protein cells. induced vitro significantly inhibited (P < 0.05). MVD decreased treated tumors CONCLUSION AdGFP/IL-24 can effectively express biologically active interleukin-24, which results growth.