Reversal effect of adenovirus-mediated human interleukin 24 transfection on the cisplatin resistance of A549/DDP lung cancer cells
作者： Mingju Xu , Xioawei Tang , Jinjin Guo , Wangbang Sun , Faqing Tang
DOI: 10.3892/OR.2017.6002
关键词:
摘要: Interleukin-24 (IL-24) is a tumor-suppressor gene that has been documented in human melanoma cells. IL-24 marked antitumor activities on various types of cancer, but its underlying mechanism remains unclear. In the present, we investigated effects (hIL-24) chemotherapy resistance lung cancer The cisplatin (DDP)-resistant carcinoma cell line A549/DDP was subjected to adenovirus-mediated transfection with gene (Ad-hIL-24). growth-inhibitory and apoptotic Ad-hIL-24 cells were observed, expression levels AKT, phosphorylated-AKT (p-AKT) P-glycoprotein (P-gp) detected. significantly decreased p-AKT P-gp, effectively inhibited growth. Furthermore, exhibited increased rate apoptosis, as well G2/M-phase arrest, following Ad-hIL-24, these treated Ad-IL-24 combined DDP when compared those or alone. These results suggest hIL-24 can reverse cells, associated involves induction apoptosis arrest through phosphoinositide3-kinase (PI3K)/AKT signaling pathway, decrease drug P-gp expression.
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