Simultaneous determination of intramolecular distance distributions and conformational dynamics by global analysis of energy transfer measurements

摘要: Fluorescence energy transfer is widely used for determination of intramolecular distances in macromolecules. The time dependence the rate a function donor/acceptor distance distribution and fluctuations between various conformations which may occur during lifetime excited state. Previous attempts to recover both distributions segmental diffusion from time-resolved experiments have been unsuccessful due extreme correlation fitting parameters. A method has developed, based on global analysis donor acceptor fluorescence decay curves, overcomes this cross-correlation allows parameters equilibrium constants be recovered with high statistical significance accuracy. Simulation studies typical reveal that static dynamic conformational information can thus obtained at single temperature viscosity.