Cyclic GMP levels and guanylate cyclase activity in pheromone-sensitive antennae of the silkmoths Antheraea polyphemus and Bombyx mori

摘要: Female sex pheromones applied to freshly isolated, living antennae of male Antheraea polyphemus and Bombyx mori led an increase cGMP. A 1:1 mixture 2 pheromone components blown for 10 sec in physiological concentrations over their antennal branches raised cGMP levels about 1.34-fold (+/- 0.08 SEM, n = 23) from a basal level 3.0 +/- 0.6 (SEM, 20) pmol/mg protein. Similarly, bombykol elicited 1.29-fold 0.13 2.7 0.5 24) No cross-sensitivity was found with respect either species. In female silkmoths, the response missing upon stimulation own respective according known lack receptor cells female. cAMP 14.2 2.9 4) protein A. 15.0 5) B. were not affected by stimulation. Within 1–60 sec, extent independent duration exposure. The pheromone- stimulated both species remained elevated at least min, i.e., much longer than potential measured single-cell recordings. Guanylate cyclase activity identified homogenates Km guanylate preferential substrate MnGTP 175 microM.(ABSTRACT TRUNCATED AT 250 WORDS)