Characterization of α-synuclein aggregation and synergistic toxicity with protein tau in yeast

摘要: A yeast model was generated to study the mechanisms and phenotypical repercussions of expression α-synuclein as well coexpression protein tau. The data show that aggregation is a nucleation–elongation process initiated at plasma membrane. Aggregation consistently enhanced by dimethyl sulfoxide, which known increase level phospholipids membranes in cells. also triggered treatment cells with ferrous ions, are oxidative stress. In addition, presented support hypothesis degradation occurs via autophagy proteasomes disturbs endocytosis. Reminiscent observations double-transgenic mice, tau synergistically toxic, exemplified inhibition proliferation. Taken together, these models recapitulate major aspects cytotoxicity, offer great potential for defining underlying toxicity synergistic actions